(CNN) — The European Union has imposed sanctions related to the crisis in Ukraine on another 15 people, bringing the total number targeted to 48.
The EU said the people are collectively responsible for actions that “undermine or threaten the territorial integrity, sovereignty and independence of Ukraine.”
The targets include Dmitry Kozak, Russia’s deputy prime minister; Russian military chief Valery Gerasimov; and pro-Russian separatists in Ukraine, including Denis Pushilin, the self-declared leader of the “Donetsk People’s Republic.”
EU foreign policy chief Catherine Ashton said she was alarmed by the worsening security situation in eastern Ukraine, and she called on Russia to take “concrete steps” in support of an international deal signed this month aimed at easing tensions.
New round of sanctions on Russia
Donetsk besieged by violence, protesters
Occupations run with military precision
Pro-Russian activists storm an administration building in the center of Luhansk, Ukraine, on Tuesday, April 29. Ukraine has seen a sharp rise in tensions since a new pro-European government took charge of the country in February.
Pro-Russia militants, armed with baseball bats and iron bars, hold flares as they attack people marching for national unity in Donetsk, Ukraine, on Monday, April 28.
Detained observers from the Organization for Security and Co-operation in Europe arrive to take part in a news conference Sunday, April 27, in the eastern Ukrainian city of Slavyansk. Vyacheslav Ponomarev, the self-declared mayor of Slavyansk, referred to the observers as “prisoners of war.”
Ukrainian troops stand guard behind a barricade made of sandbags at a checkpoint about 30 kilometers (20 miles) from Slavyansk on April 27.
Relatives and friends of a man killed in a gunfight participate in his funeral ceremony in Slavyansk on Saturday, April 26.
U.S. troops arrive at an air force base near Siauliai Zuokniai, Lithuania, on April 26. The United States is conducting military exercises in Poland, Latvia, Estonia and Lithuania. The exercises are, in part, a response to the ongoing instability in Ukraine.
Ukrainian government troops in armored vehicles travel on a country road outside the town of Svyitohirsk in eastern Ukraine on April 26.
Pro-Russia armed militants inspect a truck near Slavyansk on Friday, April 25. Russian Foreign Minister Sergey Lavrov has accused the West of plotting to control Ukraine, and he said the pro-Russia insurgents in the southeast would lay down their arms only if the Ukrainian government clears out the Maidan protest camp in the capital, Kiev.
Pro-Russia militants keep records of their duty in Slavyansk on April 25.
Ukrainian troops take position near burning tires at a pro-Russian checkpoint in Slavyansk on Thursday, April 24.
Ukrainian special forces take position at an abandoned roadblock in Slavyansk on April 24.
A Ukrainian special forces member takes position in Slavyansk.
Cossacks carry a coffin into a church in Slavyansk on Tuesday, April 22, during a funeral for men killed in a gunfight at a checkpoint two days before.
U.S. Vice President Joe Biden, left, talks with Ukrainian Prime Minister Arseniy Yatsenyuk during a meeting in Kiev on April 22.
An armed pro-Russian man stands on a street in Slavyansk on Monday, April 21.
Demonstrators attend a pro-Russian rally outside the secret service building in Luhansk on April 21.
Cars are burned out after an attack at a roadblock in Slavyansk on Sunday, April 20.
A resident inspects burnt-out cars at a roadblock on April 20.
A pro-Russian militant is seen at the roadblock near Slavyansk on April 20.
Armed pro-Russian militants stand guard at a roadblock near Slavyansk on April 20.
A masked man stands guard outside a regional administration building seized by pro-Russian separatists in Slavyansk on Friday, April 18.
People walk around barricades April 18 set up at the regional administration building that was seized earlier in Donetsk.
Russian President Vladimir Putin speaks to the media after a nationally televised question-and-answer session in Moscow on Thursday, April 17. Putin denied that Russian forces are involved in the unrest in eastern Ukraine, though he did say for the first time that Russians were active in Crimea before the peninsula voted to join the country.
Ukrainian riot police officers stand guard during a pro-Ukrainian demonstration in Donetsk on April 17.
Masked pro-Russian protesters stand guard in front of the city hall in Mariupol, Ukraine, on April 17.
U.S. Secretary of State John Kerry reaches out to shake hands with Russian Foreign Minister Sergey Lavrov at the start of a bilateral meeting to discuss the ongoing situation in Ukraine. The meeting took place April 17 in Geneva, Switzerland.
A masked gunman stands guard near tanks in Slavyansk on Wednesday, April 16.
A Ukrainian helicopter flies over a column of Ukrainian Army combat vehicles on the way to Kramatorsk, a city in eastern Ukraine, on April 16.
A masked pro-Russian gunman guards combat vehicles parked in downtown Slavyansk on April 16.
A man talks with Ukrainian soldiers as they are blocked by people on their way to Kramatorsk.
Ukrainian soldiers sit atop combat vehicles on their way to Kramatorsk.
Ukrainian Gen. Vasily Krutov is surrounded by protesters after addressing the crowd outside an airfield in Kramatorsk on Tuesday, April 15.
Pro-Russian activists guard a barricade April 15 outside the regional police building that they seized in Slavyansk.
Ukrainian troops receive munitions at a field on the outskirts of Izium, Ukraine, on April 15.
Armed pro-Russian activists stand guard on top of a Ukrainian regional administration building in Slavyansk on Monday, April 14.
A pro-Russian activist carries a shield during the mass storming of a police station in Horlivka, Ukraine, on April 14.
Russian supporters attend a rally in front of the security service building occupied by pro-Russian activists in Luhansk on April 14.
A man places a Russian flag over a police station after storming the building in Horlivka on April 14.
Men besiege the police station in Horlivka.
The Horlivka police station burns on April 14.
A Ukrainian police officer receives medical care after being attacked at the police station in Horlivka on April 14.
Pro-Russian supporters beat a pro-Ukrainian activist during a rally in Kharkiv, Ukraine, on Sunday, April 13.
Pro-Russian activists escort a man outside the secret service building in Luhansk on April 13.
Pro-Russian protesters guard a barricade in Slavyansk on April 13 outside a regional police building seized by armed separatists the day before.
Armed pro-Russian activists carrying riot shields occupy a police station in Slavyansk on April 12.
A group of pro-Russian activists warm themselves by a fire Friday, April 11, in front of a Ukrainian Security Service office in Luhansk.
Ukrainian Prime Minister Arseniy Yatsenyuk speaks April 11 during his meeting with regional leaders in Donetsk, Ukraine. Yatsenyuk flew into Donetsk, where pro-Russian separatists occupied the regional administration building and called for a referendum.
Pro-Russian young men look over the fence of a military recruitment office in Donetsk on Thursday, April 10.
Armed pro-Russian protesters occupy the Security Service building in Luhansk on April 10.
Members of the self-proclaimed government the “Donetsk Republic” vote April 10 during a meeting at the seized regional administration building in Donetsk.
Ukrainian lawmakers from different parties scuffle during a Parliament session in Kiev on Tuesday, April 8.
Workers clean up on April 8 after pro-Russian separatists and police clashed overnight in Kharkiv.
Pro-Russian protesters burn tires near a regional administration building in Kharkiv after police cleared the building on Monday, April 7.
A masked man stands on top of a barricade at the regional administration building in Donetsk on April 7.
Protesters wave a Russian flag as they storm the regional administration building in Donetsk on Sunday, April 6. Protesters seized state buildings in several east Ukrainian cities, prompting accusations from Kiev that Moscow is trying to “dismember” the country.
Pro-Russian protesters clash with police as they try to occupy a regional administration building in Donetsk on April 6.
Pro-Russian activists hold a rally in front of a Ukrainian Security Service office in Luhansk on April 6.
A young demonstrator with his mouth covered by a Russian flag attends a pro-Russia rally outside the regional government administration building in Donetsk on Saturday, April 5.
A Ukrainian soldier guards a road not far from Prokhody, a village near the Russian border, on April 5. Ukrainian and Western officials have voiced alarm about Russia’s reported military buildup on Ukraine’s eastern border.
Ukrainian cadets at the Higher Naval School embrace a friend who has decided to stay in the school during a departure ceremony in Sevastopol, Crimea, on Friday, April 4. Some 120 cadets who refused to take Russian citizenship left the school to return to Ukraine.
Soviet military veterans take part in a flower-laying ceremony at the Soviet-era World War II memorial in Sevastopol on Thursday, April 3.
Ukrainian soldiers conduct a training session on the Desna military shooting range northeast of Kiev on Wednesday, April 2.
Russian soldiers prepare for diving training in front of a Tarantul-III class missile boat Tuesday, April 1, in Sevastopol.
People pass by barricades near the Dnipro Hotel in Kiev on April 1.
People walk past a train loaded with Russian tanks Monday, March 31, in the Gvardeyskoe railway station near Simferopol, Crimea.
A Russian solder sits in a tank at the Ostryakovo railway station, not far from Simferopol on March 31.
Russian Prime Minister Dmitry Medvedev speaks about the economic development of Crimea during a meeting March 31 in Simferopol.
Members of the Ukrainian National Guard take part in military exercises on a shooting range near Kiev on March 31.
A woman cries Sunday, March 30, during a gathering to honor those who were killed during protests in Kiev’s Independence Square.
A woman and child walk past a line of police officers during a rally in Kharkiv on March 30.
Ukrainian soldiers take part in a training exercise at a military base in Donetsk on Saturday, March 29.
Demonstrators protest Friday, March 28, in Kiev, displaying police vehicles they seized during earlier clashes with authorities.
Members of the Right Sector group block the Ukrainian parliament building in Kiev on Thursday, March 27. Activists called for Interior Minister Arsen Avakov to step down after the recent killing of radical nationalist leader Oleksandr Muzychko, who died during a police operation to detain him. Muzychko and the Right Sector are credited with playing a lead role in the protests that toppled Ukrainian President Victor Yanukovych.
Ukrainian tanks are transported from their base in Perevalne, Crimea, on Wednesday, March 26. After Russian troops seized most of Ukraine’s bases in Crimea, interim Ukrainian President Oleksandr Turchynov ordered the withdrawal of armed forces from the Black Sea peninsula, citing Russian threats to the lives of military staff and their families.
Ukrainian marines wave as they leave a base in Feodosia, Crimea, on Tuesday, March 25.
Russian sailors stand on the deck of the corvette ship Suzdalets in the bay of Sevastopol on March 25.
Pro-Russian militia members remove a resident as Russian troops assault the Belbek air base, outside Sevastopol, on Saturday, March 22. After its annexation of Crimea, Russian forces have consolidated their control of the region.
Soldiers in unmarked uniforms sit atop an armored personnel carrier at the gate of the Belbek air base on March 22.
A Russian sailor holds the Russian Navy’s St. Andrew’s flag while standing on the bow of the surrendered Ukrainian submarine Zaporozhye on March 22 in Sevastopol.
Russian President Vladimir Putin signs the final decree completing the annexation of Crimea on Friday, March 21, as Upper House Speaker Valentina Matviyenko, left, and State Duma Speaker Sergei Naryshkin watch.
A Ukrainian serviceman leaves a Ukrainian military unit that Russian soldiers took control of in Perevalne on March 21.
Ukrainian border guards run during training at a military camp in Alekseyevka, Ukraine, on March 21.
Russian soldiers patrol the area surrounding a Ukrainian military unit in Perevalne on Thursday, March 20.
Pro-Russian protesters remove the gate to the Ukrainian navy headquarters as Russian troops stand guard in Sevastopol on Wednesday, March 19.
Pro-Russian forces walk inside the Ukrainian navy headquarters in Sevastopol on March 19.
A member of pro-Russian forces takes down a Ukrainian flag at the Ukrainian navy headquarters in Sevastopol on March 19.
Alexander Vitko, chief of the Russian Black Sea Fleet, leaves the Ukrainian navy headquarters in Sevastopol after pro-Russian forces took it over on March 19.
A Russian flag waves as workers install a new sign on a parliament building in Simferopol, Crimea’s capital, on March 19.
Russian military personnel surround a Ukrainian military base in Perevalne on March 19.
Nameplates on the front of the Crimean parliament building get removed Tuesday, March 18, in Simferopol.
From left, Crimean Prime Minister Sergey Aksyonov; Vladimir Konstantinov, speaker of the Crimean parliament; Russian President Vladimir Putin; and Alexei Chaly, the new de facto mayor of Sevastopol, join hands in Moscow on March 18 after signing a treaty to make Crimea part of Russia.
Demonstrators hold a Crimean flag at Lenin Square in Simferopol on March 18.
Ukrainian soldiers stand guard at a checkpoint near Strilkove, Ukraine, close to Crimea on Monday, March 17.
Former boxer and Ukrainian politician Vitali Klitschko addresses reporters in Kiev on March 17.
Ukrainian troops stand guard in front of the Ukrainian Parliament building in Kiev on March 17.
A Ukrainian man applies for the National Guard at a mobile recruitment center in Kiev on March 17.
Civilians walk past riot police in Simferopol on March 17.
A Ukrainian soldier stands on top of an armored vehicle at a military camp near the village of Michurino, Ukraine, on March 17.
Policemen stand guard outside the regional state administration building in Donetsk during a rally by pro-Russia activists March 17.
Armed soldiers stand guard outside a Ukrainian military base in Perevalne on March 17.
A man holds a Crimean flag as he stands in front of the Crimean parliament building in Simferopol on March 17.
Crimeans holding Russian flags celebrate in front of the parliament building in Simferopol on Sunday, March 16.
A Ukrainian police officer tries to shield himself from a road block thrown by pro-Russia supporters in Kharkiv on March 16.
Pro-Russia demonstrators storm the prosecutor general’s office during a rally in Donetsk on March 16.
A woman leaves a voting booth in Sevastopol on March 16. See the crisis in Ukraine before Crimea voted
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Photos: Crisis in Ukraine
She warned that if necessary, the European Union “will look at possible additional individual measures” related to the crisis.
Ashton also condemned an attack on the mayor of Kharkiv on Monday and the continued detention by pro-Russian militants of a team of military observers from the Organization for Security and Co-operation in Europe.
“All persons still illegally detained by armed groups in eastern Ukraine need to be immediately released,” she said.
On Tuesday, pro-Russian activists in eastern Ukraine took control of the regional government headquarters in Luhansk. Video shows them as they approach the building, smash doors, wave flags and chant “Russia! Russia!”
Pro-Russia activists also tried to break into that city’s police headquarters by ramming a truck at the entrance, according to state-run news agency Ukrinform.
A local reporter for Ukrinform, who was on the scene at police headquarters, described a fight, involving an explosion and gunfire. The activists demanded that police surrender their weapons and exit headquarters. They also claimed that the Luhansk police chief had submitted his resignation and that a new “people’s police” chief had been “assigned,” though not everyone in the crowd agreed with the selection, the news agency reported.
On his official website, Acting President Oleksander Turchinov said the events in eastern Ukraine “illustrated inactivity, helplessness, and sometimes criminal betrayal of the law enforcement agencies in the Donetsk and (Luhansk) regions.”
He said, “It is hard to admit, but it is true. The vast majority of the law enforcement officials in the East are not able to fulfill their obligations to protect our citizens.”
New heads of security have been appointed in Donetsk and Luhansk, he said.
Western nations accuse Moscow of supporting the separatist gunmen who are occupying official buildings in cities across the region and are holding the OSCE team hostage.
Russia disputes that claim, saying it has no direct influence over the pro-Russian activists.
Russia: ‘Aren’t you ashamed?’
In a statement on its website Tuesday, the Russian Foreign Ministry said the EU sanctions “cannot but cause rejection” and show a lack of comprehension of Ukraine’s internal political situation.
“Instead of making the Kiev faction sit at the negotiating table with southeastern Ukraine, our partners follow Washington’s lead with new unfriendly gestures regarding Russia,” it said.
The EU action is “a direct invitation for local neo-Nazis to continue to promote anarchy and outrages regarding the civilians of the southeast,” it said, repeating Russia’s contention that ultranationalist groups are behind the unrest in Ukraine.
The statement concluded with the question, “Aren’t you ashamed?”
On Monday, Russia promised a painful response to sanctions imposed by the United States.
Sergei Ryabkov, Russia’s deputy minister for foreign relations, called the U.S. measures “meaningless, shameful, and disgusting.”
“It will only intensify all the processes in Ukraine which it intends to change or stop,” Ryabkov told CNN, speaking English. “The U.S. does literally nothing to impress its cronies and clients in Kiev on whom there is full responsibility for constant deterioration of the situation in Ukraine. This is what needs to be changed and not the policy of Russia.
“A response of Moscow will follow, and it will be painfully felt in Washington.”
Observers detained in eastern Ukraine
Living on the edge in eastern Ukraine
‘Chaotic’ situation unfolding in Ukraine
Russia has not specified what measures it will impose against Western interests.
According to Russia’s state-run ITAR-Tass news agency, Russian President Vladimir Putin said his government has proposed imposing sanctions against the EU and the United States, but that he has not yet authorized them.
“The government of the Russian Federation has already proposed some retaliatory steps. I think there’s not a need,” the President reportedly told journalists.
“We would not like to resort to some steps — retaliatory measures. I hope there will be no need,” Putin said, according to ITAR-Tass.
Ukraine crisis: The full sanctions list
U.S. sanctions
But U.S. Secretary of State John Kerry appeared unfazed. He pledged Tuesday in an address to the Atlantic Council, a Washington think tank, that NATO would stand united to defend its members’ territorial integrity.
In its latest round of sanctions, the United States targeted seven Russian government officials and 17 companies linked to Putin.
The White House said the seven Russians, including two from Putin’s inner circle, are subject to a freeze on any assets they may hold in the United States and a ban on U.S. travel.
The two seen as closest to Putin are Igor Sechin, chairman of Russian oil giant Rosneft, and Sergey Chemezov, director general of Rostec, a state-owned industrial conglomerate in Russia. The companies the United States named are linked to officials and oligarchs who were designated last month, and the list included neither Rosneft itself nor gas exporter Gazprom.
In addition, the United States will deny export license applications for any high-technology items that could contribute to Russian military capabilities. The Commerce and State departments will revoke any existing export licenses that meet these conditions, the White House said in a statement.
The move, Obama told reporters in Manila, Philippines, was intended to spur Putin to “walk the walk, not just talk the talk” in resolving the crisis in Ukraine.
If the latest round of sanctions does not work, the next phase could target economic sectors like banking, Obama said.
In all, the United States and European Union have imposed asset freezes and travel bans on 66 individuals, most of them senior Russian officials. The United States has sanctioned 18 companies in total.
Analysis: Sanctions-lite won’t move Russia
‘Stolen assets’
Meanwhile, Britain is hosting a two-day international meeting aimed at helping Ukraine’s government recover stolen assets, following claims of widespread corruption within the government of ousted Ukrainian President Viktor Yanukovych.
UK Home Secretary Theresa May, U.S. Attorney General Eric Holder and the head of the Ukrainian delegation, acting Prosecutor General Oleh Makhnitskyi, are all taking part in the London forum.
“What we have committed to do is to persevere, to follow leads wherever we can find them,” said Holder.
Makhnitskyi said investigators were focused on years when Yanukovych and his associates were in charge. “The Ukrainian society already demands results from the government,” he said.
Yanukovych fled to Russia in February after months of street protests that were sparked by his decision to drop closer trade ties with Europe and turn instead toward Moscow.
In Ukraine, presidential elections have been called for May 25. But Oleg Tsarev said he had dropped out of the race.
On his website, he said that he received death threats when he arrived in Kiev and that Ukrainian National Television turned down his request to participate in televised debates.
“In this regard, I have decided to withdraw my candidacy, because in such circumstances, being a candidate is not helpful,” Tsarev said. “We will seek other channels and opportunities to convey our thoughts and positions to residents of the central and western Ukraine.”
Ukraine crisis: Who will blink first, Vladimir Putin or the West?
Kharkiv mayor being treated in Israel
Kharkiv Mayor Gennady Kernes, who underwent surgery after being shot in the back Monday, is being treated in Israel, a hospital official said Tuesday.
Kernes arrived overnight at Elisha Hospital in Haifa, the hospital’s chief accountant Jacob Karwasser told CNN.
The mayor was in stable condition, he said, and has family members with him.
It was not immediately clear who was responsible for the shooting. Police said an investigation was under way.
Mayor of Ukrainian city shot; West prepares more sanctions
CNN’s Nick Paton Walsh, Tom Watkins, Phil Black, Victoria Butenko, Stephanie Halasz, Carol Jordan, Alla Eshchenko, Alexander Felton and Brooke Bowman contributed to this report.